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. 2020 Apr 7;12:92. doi: 10.3389/fnagi.2020.00092

Figure 5.

Figure 5

Western blot detection and densitometric analysis of Aβ using 6E10 antibody (A) Representative Western blot of Aβ using 6E10 antibody. Freshly prepared synthetic human Aβ1–42 (23 μM) was added to serum-free media (SFM; lane 1), SFM that had been conditioned by incubation with C6 glial cells (CM; lane 2), or CM that had been treated with MPA (100 nM or 10 μM) for 48-h (lanes 3 and 4). The mixture was then incubated for 24 h at 37°C, and residual Aβ was analyzed by Tris-Glycine—Western blotting. Incubation of Aβ1–42 with CM significantly decreased Aβ levels. MPA treatment attenuated this effect. (B–E) Densitometric analysis shows that MPA-treated CM samples induced significant differences in Aβ species. Incubation of Aβ1–42 with CM significantly decreased Aβ levels. MPA treatment attenuated this effect. Results are representative of three independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001.