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. 2020 Mar 12;3(2):334–344. doi: 10.1021/acsptsci.0c00013

Figure 5.

Figure 5

Impaired mTOR signaling in 12 month old APPswe/PS1ΔE9 mice was normalized by 24 weeks of CTEP treatment. Representative Western blots and quantification of folds change in (A) Akt-pS473, (B) mTOR-pS2448, and (C) P70S6K1-pT389 with the corresponding loading controls in brain lysates from age-matched wild-type (wt) and APPswe/PS1ΔE9 (APP) mice after either a 24 week or 36 week treatment with either vehicle or CTEP (2 mg/kg). Akt-pS473 was normalized to total Akt, mTOR-pS2448 was normalized to mTOR, and p70S6K1-pT389 was normalized to vinculin (n = 5 for each group). P70S6K1-pT389 and p62 (Figure 4D) were probed on the same blot. Values represent mean ± SD and expressed as a fraction of the age-matched, vehicle-treated wt value. *P < 0.05 versus age-matched, vehicle-treated wt values. Statistical significance was assessed by two-way ANOVA and Fisher’s LSD comparisons.