Figure 1.

(A) Amino acid sequence alignment of AM and related peptides from humans. (B) Amino acid sequences of peptide fragments used during this study. (C) Amino acid sequences of alanine-substituted analogues used in this study, substitutions are highlighted in orange. All sequence alignments performed in Geneious 11, using in-built ClustalW alignment and amino acid comparison. Unless otherwise noted elsewhere, there is a disulfide bond between C16 and C21 of the AM peptides (or the corresponding conserved cysteines in other peptides), and all the peptides are amidated at the C-terminus.