Table 5. cAMP Signalling Parameter Estimates of His1- and Phe1-Containing Chimeric Ligands in INS-1 832/3 Cells^a.

	pEC50 (M)	Emax (%)
GLP-1	8.6 ± 0.2	100
GLP-1-phe1	7.3 ± 0.1*,#	100
Chi1	8.7 ± 0.2	100
Chi1-phe1	7.5 ± 0.1*,#	100
Chi2	9.2 ± 0.2*	100
Chi2-phe1	8.6 ± 0.1#	100
Chi3	9.3 ± 0.2*	100
Chi3-phe1	8.4 ± 0.1#	100
Ex-ala2	9.4 ± 0.2*	100
Ex-ala2-phe1	8.8 ± 0.1#	100
Ex4	9.3 ± 0.2*	100
Ex-phe1	8.4 ± 0.2#	100
GLP-1-gly2	8.3 ± 0.1	100
GLP-1-gly2-phe1	6.4 ± 0.1*,#	100

^aMean ± SEM signaling parameter estimates from data shown in Figure 3, n = 5 experimental repeats. Signaling parameter estimates were determined from 3-parameter fitting. All compounds are full agonists for cAMP in this cell model, so E_max was globally constrained to 100%. *p < 0.05 versus GLP-1, and #p < 0.05 for Phe1 ligand versus corresponding His1 ligand, determined by one-way ANOVA with Tukey’s test.