Table 2.

Primers and probes used in the study

Product	Primer	Sequence	Fragment	References
All herpesviruses
Pan herpesvirus	DFA (For)	GAYTTYGCNAGYYTNTAYCC*	700 bp	VanDevanter et al. (1996)
	ILK (For)	TCCTGGACAAGCAGCARNYSGCNMTNAA*
	KG1 (Rev)	GTCTTGCTCACCAGNTCNACNCCYTT*
Pan herpesvirus (nested PCR)	TGV (For)	TGTAACTCGGTGTAYGGNTTYACNGGNGT*	250 bp
	IYG (Rev)	CACAGAGTCCGTRTCNCCRTADAT*
Equid herpesvirus 1 (EHV‐1)†
glycoprotein B (gB)	For	61722‐CACTTCCATGTCAACGCACT‐61741	869 bp	Designed for this study
	Rev	62591‐TCGACTTTCTTCTCGGTCCA‐62572
DNA polymerase (POL)	For	54885‐ACCTCCGGAGGCAAAGTTCA‐54904	709 bp
	Rev	54195‐TTCGCCCCGTTGAGCGACAC‐54214
EHV‐4†
gB	For	61314‐CATGTCTAAAGACTCGACAT‐61333	1369 bp	Designed for this study
	Rev	62664‐GATTGGTATTATGGTTTGCG‐62683
POL	For	54660‐CATCACAGTACACTTTTGGG‐54679	843 bp
	Rev	53836‐ACTATAAGCTACTGTGTTTT‐53855
EHV‐2†
POL	For	36400‐GCGCGTGTTGCGCGAGTACT‐36419	1270 bp	Designed for this study
	Rev	37651‐GGTGCAGGCACAGCCTGTCT‐37670
EHV‐5†
POL	For	32972‐AAGGGTTTTGAAACAATACA‐32991	1115 bp	Designed for this study
	Rev	34068‐GAACTTTCCTTGTTGCCCGA‐34087
qPCR
EHV‐1 gB	For	CATACGTCCCTGTCCGACAGAT		Hussey et al. (2006)
	Rev	GGTACTCGGCCTTTGACGAA
	Probe	6FAM‐TGAGACCGAAGATCTCCTCCACCGA‐BHQ1
EHV‐4 gB	For	CGCAGAGGATGGAGACTTTTACA		Pusterla et al. (2005)
	Rev	CATGACCGTGGGGGTTCAA
	Probe	6FAM‐CTGCCCGCCGCCTACTGGATC‐TAMRA
EHV‐2 gB	For	AGGACTACTACTATGTCAG		Dunowska et al. (2011)
	Rev	ATGGTCTCGATGTCAAACAC
	Probe	6FAM‐TGACATACCCACCCTACACACCATGA‐BHQ1
EHV‐5 gB	For	ATGAACCTGACAGATGTGCC		Dynon et al. (2001)
	Rev	CACGTTCACTATCACGTCGC
	Probe	6FAM‐TCCATCCACGATGGCAGGGA‐BHQ1

^*Bold letters indicate amino acid residues included in the primers.

^†Numbers before and after primer sequences represent their positions in the genome.