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L02 (a) and HepG2 (b) cells were cultured in 6-well plates for 48 hours, then apoptosis of L02 cells and HepG2 cells was detected by flow cytometry. TiO2 increased the percentage of apoptotic liver cancer cells. Annexin V-positive cells were considered as apoptotic liver cancer cells. Cells incubated with 0 µg/mL TiO2 were used as a control. *P < 0.05 and **P < 0.01 vs. 0 µg/mL (control). AAD, 7-amino-actinomycin D.
