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. 2020 Apr 7;14:86. doi: 10.3389/fncel.2020.00086

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Copyright © 2020 Minassian, Green, Diedenhofen, Vogel, Hess, Stoeber, Radmilovic, Wiedermann, Kloppenburg and Hoehn.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic presentation of the project timeline. Time of stroke induction by distal occlusion of the middle cerebral artery (dMCAO) serves as day zero for the MRI scans. Human neural stem cells (hNSCs) were intracortically implanted 7 days later. Repetitive measurements of bioluminescence imaging (BLI) for graft vitality monitoring and resting-state fMRI (rsfMRI) for functional connectivity networks of the brain are indicated on a separate time ray with day 0 at the day of implantation. At 12 weeks after stroke induction, some of the mice were sacrificed to process brains for histology (IHC). Weeks after stroke and days after engraftment, respectively, are marked along the timeline.