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. Author manuscript; available in PMC: 2020 Apr 14.
Published in final edited form as: Brain Behav Immun. 2019 Jul 17;81:545–559. doi: 10.1016/j.bbi.2019.07.015

Fig. 2.

Fig. 2.

Metabolites of PER pesticide is detected as protein adducts in the brain and liver homogenates. (A) A schematic of PER metabolites. (B) Detection of precursor and product ion of 3-PBA lysine (343.2) and its product ions (197.06) in the brain and liver homogenates from a GWI mouse sample. (C and D) Using LC-MS and LC-MS/MS, we detected 3-PBA (m/z 213.055 at 3 min) and its PRM product ion (m/z 213.1 > 93.033 at that same time) in the brain and liver (mean ± SE (n = 4 or 5/ group). While only protein-conjugated 3-PBA recovered after hydrolysis was detected in the brain, free 3-PBA and protein-conjugated 3-PBA were detected in the liver at 1.5 months post-exposure. (E and F) Peak plots show absence of 3-PBA (m/z 213.055 at 3 min) and its transition ion (m/z 93.033) ions in control mice. These peak plots show presence of 3-PBA internal standard 219.1 at 3 min and its transition ion (m/z 93.033) ions in control mice.