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. 2020 Apr 7;10:383. doi: 10.3389/fonc.2020.00383

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Copyright © 2020 Wang, Wang, Wu, Li, Huang, Zhou and Cui.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cx43 expression and GJ function of HCT116 and CT26 cells, including parental, control-shRNA, and Cx43-shRNA transfected cells. RES, resveratrol-treated cells. All other groups were compared with the Parental group using one-way ANOVA. *P < 0.05 represents a significant difference from values in the Parental group. SEMs are shown as error bars. (A1) Western blot images of Cx43 and phospho-Cx43 (Ser-368). β-Actin is used as a loading control. The full-length blots are shown as Figure S7A. (A2,A3) Bar diagrams of densitometric analysis. Protein expression level is normalized by β-actin. (B) GJ function analysis by “Parachute” dye-coupling assay. Scale bars are 20 μm. Columns show the mean ± SEM. All images of “Parachute” dye-coupling assay are shown as Figure S1.