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. 2020 Apr 7;10:383. doi: 10.3389/fonc.2020.00383

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Copyright © 2020 Wang, Wang, Wu, Li, Huang, Zhou and Cui.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunoprecipitation analysis of Akt and Cx43 in parental HCT116 and CT26 cells. Cell lysates were subjected to immunoprecipitation with anti-Akt or anti-Cx43 antibodies. Then immunoprecipitated proteins were examined by western blotting with anti-Cx43 or anti-Akt antibodies, respectively. The input of cell total protein was used as a positive control and rabbit IgG was used as a negative control. Molecular weight of Akt and IgG heavy chain is similar, so they are hardly to be discriminated. SDS-PAGE of immunoprecipitation analysis is shown as Figure S5. (A) IB: Cx43; IP: Akt. (A1) Non-treated cells. (A2) Resveratrol-treated cells. (A3) Cetuximab-treated cells. (A4) Cetuximab + Resveratrol-treated cells. (B) IB: Akt; IP: Cx43. (B1) Non-treated cells. (B2) Resveratrol-treated cells. (B3) Cetuximab-treated cells. (B4) Cetuximab + Resveratrol-treated cells.