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. 2019 Jun 4;22(2):192–199. doi: 10.4103/aja.aja_44_19

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Copyright: ©The Author(s)(2019)

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Effects on inositol 1,4,5-trisphosphate/BioPORTER^®-induced acrosome reaction with or without calcium in the culture medium. IP3/BioPORTER^® induced acrosome reactions in the regular culture medium containing 2.4 mmol l^-1 calcium chloride (P < 0.001). It also induced an acrosome reaction in calcium-deficient culture medium (P = 0.018). However, the acrosome reaction induced by IP3/BioPORTER^® was significantly increased when the culture medium contained calcium (P < 0.001). As a negative control, transfer of β-galactosidase into spermatozoa by BioPORTER^® had no effect on the acrosome reaction (P = 1.000). PBS/BioPORTER^® was also used to eliminate the possible impact of the transport tool and had no effect on the acrosome reaction (P = 0.606). The data are expressed as the mean ± s.d., ^*P < 0.05, ^***P < 0.001, n = 3. IP3: inositol 1,4,5-trisphosphate; PBS: phosphate-buffered saline; s.d.: standard deviation.