Figure 4.

Spermatogonial proliferation in P7 Mtor KO testes. Representative images of costained GFRA1 (green), KI67 (red) and DAPI (blue) in the testes of P7 (a) control and (a') Mtor KO mice. Representative images of costained PLZF (green), BrdU (red) and DAPI (blue) in the testes of P7 (b) control and (b') Mtor KO mice. Representative images of costained STRA8 (green), BrdU (red) and DAPI (blue) in the testes of P7 (c) control and (c') Mtor KO mice. Scale bars = 50 μm. Quantification of the proportion of (d) GFRA1⁺/KI67⁺, (e) PLZF⁺/BrdU⁺ and (f) STRA8⁺/BrdU⁺ germ cells in the testes of P7 control and Mtor KO mice. ^*P < 0.05; ^**P < 0.01.