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. Author manuscript; available in PMC: 2021 Feb 12.
Published in final edited form as: Cell Host Microbe. 2019 Dec 31;27(2):225–237.e8. doi: 10.1016/j.chom.2019.12.002

Figure 4. Vesiculation accelerates depletion of OmpT from the OM.

Figure 4.

Samples of whole cell lysates, OM preparations and OMVs were isolated from WT, ΔyrbE, and yrbE* at times indicated after transition from minimal medium M9 to M9ToxR↑. Equivalent amounts of total proteins were resolved electrophoretically and representative immunoblots detected OmpU, OmpT and RpoA in case of whole cell lysates (left) or OmpU and OmpT in case of OM preparations (center) and OMVs (right). Coomassie-stained SDS gels executed in parallel additionally controlled loading of equal protein amounts in all lanes. See also Figure S3.