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. Author manuscript; available in PMC: 2021 Mar 9.
Published in final edited form as: ACS Biomater Sci Eng. 2020 Feb 5;6(3):1408–1417. doi: 10.1021/acsbiomaterials.9b01873

Figure 5.

Figure 5.

Microstructural characterization of collagen-based matrices. (A) Confocal projections of 3 and 6 mg/mL collagen matrices with added hrTGII and HA (scale Bar = 20 μm). (B) Average matrix pore size determined by the nearest obstacle method. hrTGII did not alter the pore size of 3 mg/mL collagen matrices and slightly increased the pore size of 6 mg/mL collagen matrices. HA increased the average matrix pore size for both collagen concentrations. (C) Alignment of collagen fibers quantified with alignment index. hrTGII increased the alignment of 3 mg/mL collagen matrices but not for 6 mg/mL matrices. Addition of HA did not alter the alignment index at either collagen concentration. (D) Mean fiber radius quantified through 3-D skeletonization. Addition of HA significantly increased the radius of collagen fibers at both collagen concentrations. hrTGII did not alter the radius of the collagen fibers.