Fig 4. Timing of SMURF appearance using only flies that satisfied our criteria for being a Smurf.

Most flies appeared to be Smurfs on the day they are found dead or 1 day before death. Of the D. melanogaster that became a Smurf, a majority of them did so on the day of their death or one day before. Bars are simultaneous 95% confidence intervals. Dye 1: SPS Alfachem Blue, Dye 2: Sigma Aldrich, Dye 3: Spectrum Blue, Dye 4: Flavors and Color Blue, Dye 5: Chemistry Connection Blue, and Dye 6: Electric Blue.