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. 2020 Apr 14;15(4):e0231796. doi: 10.1371/journal.pone.0231796

Fig 6. Elafin promotes fibrogenesis in human colonic fibroblasts.

Fig 6

(A) The human colonic CCD-18Co fibroblasts were incubated with 100μl/ml (10%) of human sera from normal, stricturing CD, and non-stricturing CD patients in serum-free DMEM for 24 hours. (B) The human colonic CCD-18Co fibroblasts were incubated with 100μl/ml (10%) of human sera from high elafin CD group (>8000pg/ml) and low elafin CD group (<8000pg/ml) in serum-free DMEM for 24 hours. (C) Serum-starved CCD-18Co fibroblasts were pretreated with 15μg/ml of anti-elafin neutralizing antibody or control antibody for 30 minutes, followed by exposure to (100μl/ml) human sera from normal and stricturing CD patients for 24 hours. Six serum donors per group. (D) Serum-starved CCD-18Co fibroblasts were exposed to elafin (0.1–3 μg/ml) for 24 hours. Pro-COL1A1 protein levels in cell lysates were determined by ELISA. (E) Serum-starved primary human intestinal fibroblasts from CD patients were exposed to elafin for 24 hours. mRNA expression was determined by real-time RT-PCR.