Table 2.
Confirmation of COVID-19 by RT-PCR, whole genome sequencing, and virus isolation
|
Samples |
RT-PCR targets |
Virus sequence | Virus isolate titre (PFU/mL) | |||||
|---|---|---|---|---|---|---|---|---|
| Day post symptom onset | Nature | RdRp14 (cycle threshold) | E gene14(cycle threshold) | RdRp-IP1(cycle threshold) | GAPDH(cycle threshold) | |||
| Patient 1 | 6 | Nasopharyngeal swab | 28·5 | 27·3 | 26·7 | 27·4 | EPI_ISL406597* | No |
| Patient 2 | 9 | Nasopharyngeal swab | Negative | 34·7 | 33·0 | 27·1 | No | No |
| Patient 3 | 7 | Nasopharyngeal swab | Negative | 30·3 | 29·2 | 25·7 | Partial | No |
| Patient 3 | 7 | Bronchoalveolar lavage | Negative | 27·4 | 27·3 | 24·7 | Partial | No |
| Patient 4 | 2 | Nasopharyngeal swab | 23·6 | 22·8 | 23·0 | 26·5 | EPI_ISL406596 | 6·25 × 105 |
| Patient 5 | 2 | Nasopharyngeal swab | 24·3 | 20·0 | 19·3 | 25·6 | EPI_ISL408430 | 3·0 × 107 |
COVID-19=coronavirus disease 2019. PFU=plaque-forming unit.
*
Sequence number in Global Initiative on Sharing All Influenza Data.