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. Author manuscript; available in PMC: 2020 Apr 14.
Published in final edited form as: Biochem J. 2019 Dec 23;476(24):3817–3834. doi: 10.1042/BCJ20190743

Figure 1.

Figure 1.

MS/MS spectra of selected crosslinked peptides detected in human lenses.

a) Tandem mass spectrum for the AQP0/AQP0 crosslinked peptide detected in the tryptic digest of UIF. This crosslinked peptide was detected in the majority of the lenses analysed. The tandem mass spectrum shows the crosslink between Asn 246 (GAKPDVSN*) and Lys 228 (LK*SISER). b) Tandem mass spectrum for αA/αB-crystallin crosslinked peptide. The tandem mass spectrum shows the crosslink between αA Asn 101 (HN*) and αB Lys 150 (K*QVSGPER). The loss of NH3 in this spectrum is consistent with that observed under gas-phase conditions [45]