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. Author manuscript; available in PMC: 2020 Apr 14.
Published in final edited form as: Biochem J. 2019 Dec 23;476(24):3817–3834. doi: 10.1042/BCJ20190743

Figure 3. Decomposition of Ac-YHNEF at pH 6.7.

Figure 3.

a) HPLC profile of Ac-YHNEF prior to incubation in 100mM phosphate buffer, pH 6.7 b) HPLC profile of Ac-YHNEF after 21 days of incubation at 60°C in 100mM phosphate buffer, pH 6.7. Peaks were identified by co-elution with synthetic peptide standards and MS/MS. Insert; expanded region of boxed HPLC trace. Detection at 216nm.