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. Author manuscript; available in PMC: 2020 Apr 14.
Published in final edited form as: Biochem J. 2019 Dec 23;476(24):3817–3834. doi: 10.1042/BCJ20190743

Figure 4. A comparison of Asn deamidation and cleavage using Ac-YHNEF and Ac-YSNGF.

Figure 4.

a) Deamidation of peptides at pH 5.0, 6.7 and 7.4. Deamidation of Asn to Asp was calculated using relative HPLC peak areas (see Fig 3); b) Cleavage of the same peptides at pH 5.0, 6.7 and 7.4. c) The ratio of cleavage to deamidation for each peptide after seven days. d) The ratio of C-terminal Asp-amide to Asn formed from original peptides at pH 6.7 and 7.4. All data are for peptides incubated for 7 days at 60°C. Values are n=3 +/− SD.