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. Author manuscript; available in PMC: 2020 Apr 14.
Published in final edited form as: Biochem J. 2019 Dec 23;476(24):3817–3834. doi: 10.1042/BCJ20190743

Figure 7. Ac-YHD-amide readily forms a crosslink with PE.

Figure 7.

a) Crosslink formation following incubation of Ac-YHD-amide or Ac-YHN with PE at pH 6.7 and 7.4 at 60 °C. b) Bar graphs indicating the rate of Ac-YHD formation at pH 6.7 and 7.4 from Ac-YHD-amide. When the amount of crosslinked adduct is included, the sum of the Ac-YSD and PE adduct approximately equals that of the Ac-YSD in the absence of PE. Experiments were conducted in triplicate at 60°C, pH 6.7 with a five-fold molar excess of PE.