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. Author manuscript; available in PMC: 2020 Apr 14.
Published in final edited form as: Biochem J. 2019 Dec 23;476(24):3817–3834. doi: 10.1042/BCJ20190743

Figure 9. Cleavage at Asp compared with the rate of cleavage at Asn.

Figure 9.

The rate of cleavage of Ac-YHNEF and Ac-YHDEF as a function of pH. Cleavage of Ac-YHNEF was calculated as the sum of the peak areas of Ac-YHD, Ac-YHD-amide, Ac-YHN, and Ac-YH(Asu) by comparison to the amount of original peptide injected on HPLC. Ac-YHDEF cleavage was calculated as above using only Ac-YHD peak area. Each time point n=3. All peak areas were calculated from absorbance at 280nm. Experiments were conducted in triplicate at 60°C.