TABLE 4.
Primers used for qPCR analysesa
| Primer | Sequence (5′–3′) |
|---|---|
| miR-27a-SL-primer | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGCGGAAC |
| miR-27b-SL-primer | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGCAGAAC |
| U6-SL-primer | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTATGGAAC |
| mmu-miR-27-FP | ACACTTTCACAGTGGCTAA |
| U6-FP | CTGCGCAAGGATGACACGCA |
| Universal RP | GTGCAGGGTCCGAGGT |
| mHmgcr-FP | GAGAATGCAGAGAAAGGTG |
| mHmgcr-RP | GGCGAATAGACACACCAC |
| mβ-Actin-FP | CTTCTTTGCAGCTCCTTCGTT |
| mβ-Actin-RP | TTCTGACCCATTCCCACCA |
| rHmgcr-FP | ACCTGCTGCCATAAACTGGAT |
| rHmgcr-RP | ACCACCTTGGCTGGAATGAC |
| rβ-Actin-FP | GCTGTGCTATGTTGCCCTAG |
| rβ-Actin-RP | CGCTCATTGCCGATAGTG |
| hHmgcr-FP | TCGGTGGCCTCTAGTGAGAT |
| hHmgcr-RP | TGTCCCCACTATGACTTCCC |
| mMvk-FP | GGTGTGGTCGGAACTTCCC |
| mMvk-RP | CCTTGAGCGGGTTGGAGAC |
| mFdft1-FP | ATGGAGTTCGTCAAGTGTCTAGG |
| mFdft1-RP | CGTGCCGTATGTCCCCATC |
| mHmgcs1-FP | TTGAGGAGTCTGGGAATACAG |
| mHmgcs1-RP | CATATCGTCCATCCCAAGAG |
| mGgps1-FP | GTCATCTCCAGCAGTTCCTTC |
| mGgps1-RP | TCATCTCGTCCAGCATCTTC |
| mScarb1-FP | CAGGTGCTCAAGAATGTCC |
| mScarb1-RP | TTTGTCTGAACTCCCTGTAG |
| mMvd-FP | ATGGCCTCAGAAAAGCCTCAG |
| mMvd-RP | TGGTCGTTTTTAGCTGGTCCT |
a
Abbreviations: FP, forward primer; RP, reverse primer; SL, stem-loop.