Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Recombinant DNA reagent (X. laevis) | pFastbac1-FLAG-xFANCI | (Klein Douwel et al., 2014) | Gift from Puck Knipscheer | |
| Recombinant DNA reagent (X. laevis) | pFastbac1-StrepII-xFANCD2 | (Klein Douwel et al., 2014) | Gift from Puck Knipscheer | |
| Recombinant DNA reagent (H. sapiens) | pFL-EGFP-His-hFANCI | (Tan et al., 2020a) | ||
| Recombinant DNA reagent (H. sapiens) | pFastbac1-FLAG-hFANCD2opt | (Tan et al., 2020a) | RRID:Addgene_ 134904 | Gift from Angelos Constantinou |
| Recombinant DNA reagent (H. sapiens) | pFL/pSPL-EGFP-FLAG-B-L-100 | (van Twest et al., 2017) | Codon optimized FANCB | |
| Recombinant DNA reagent (H. sapiens) | pFL-MBP-C-E-F | (van Twest et al., 2017) | ||
| Recombinant DNA reagent (H. sapiens) | pGEX-KG-GST-UBE2T | (van Twest et al., 2017) | Codon optimized | |
| Recombinant DNA reagent (E. coli) | pet16b-Avi-ubiquitin_rbs_BirA | (Tan et al., 2020a) | RRID:Addgene_134897 | |
| Recombinant DNA reagent (E. coli) | pSRK2706-GST-HRV-3Cprotease | (Raran-Kurussi and Waugh, 2016) | RRID:Addgene_78571 | A gift from David Waugh |
| Recombinant DNA reagent (E. coli) | pUC19 plasmid | New England BioLabs | N3041S | |
| Strain, strain background (E. coli) | BL21 (DE3) | Agilent Technologies | 200131 | |
| Cell line (Spodoptera frugiperda) | Sf9 | Thermo Fisher Scientific | RRID:CVCL_0549 | Maintained in Sf-900 II SFM |
| Cell line (Trichoplusia ni) | High Five | Thermo Fisher Scientific | RRID:CVCL_C190 | Maintained in Sf-900 II SFM |
| Antibody | Rabbit polyclonal antibodies against StrepII | Abcam | RRID:AB_76949 | one in 3000 dilution |
| Antibody | Rabbit polyclonal antibodies against FANCI | Abcam | RRID:AB_74332 | one in 3000 dilution |
| Antibody | Rabbit polyclonal antibodies against FANCD2 | Abcam | RRID:AB_10862535 | one in 3000 dilution |
| Antibody | Mouse monoclonal antibodies against FLAG | Aviva Biosciences | RRID:AB_10884242 | one in 3000 dilution |
| Peptide, recombinant protein | FLAG peptide | Sigma-Aldrich | F3290 | |
| Peptide, recombinant protein | Recombinant Human His6-Ubiquitin E1 Enzyme carrier free | Boston Biochem | E-304–050 | |
| Peptide, recombinant protein | Ubiquitin and associated mutant variants | Boston Biochem | U-110H, UM-I44A, UM-D58A, UM-F4A, UM-L73P, UM-K11R | |
| Commercial assay or kit | TNT T7 Quick Coupled Transcription/Translation System | Promega Corporation | L1170 | |
| Commercial assay or kit | Anti-FLAG-M2 affinity gel | Sigma Aldrich | RRID:AB_10063035 | |
| Chemical compound, drug | EasyTagL-[35S]-Methionine | PerkinElmer Life Sciences | NEG709A500UC | |
| Software, algorithm | XMIPP | (de la Rosa-Trevín et al., 2013) |