Figure 2. Loss of Unc-4 function results in behavioral defects in adult flies.
(A–H) Still images from recorded videos showing the behavioral defects observed in unc-4 mutants (A, C, E, and G) and the rescue of these defects with uncDBD- tubAD driven UAS-unc-4 transgene (B, D, F, and H). Images from control animals not shown as they were virtually identical to the images from rescued animals. Wings of unc-4 mutants are locked in an erect position (A); they fail flying in a tethered flight assay (C). Restoring Unc-4 function enables mutant animals to rest their wings in tucked position (B) and fly in the flight assay (D). Mutant animals fail to bring their three legs together for rubbing off dust (E) and rescue animals regain this three-leg rubbing behavior (F). (G–H) Restoring Unc-4 expression also improves climbing defects of unc-4 mutants. Positions of mutant and rescued animals after bang-induced climbing shown; the image was reproduced from Video 5. (I) Percentage bar graphs showing the quantification of unc-4 mutant phenotypes (orange bars) and their rescue with uncDBD- tubAD driven UAS-unc-4 (green bars) or a BAC transgene (PBac(DC335)) containing the unc-4 locus (magenta bars). See methods for detailed explanations for the assays quantified here. See also Videos 1–7.