Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Guinea pig anti-Sens polyclonal | PMID:10975525 | 1:1000 dilution | |
| Antibody | Rabbit anti- GABA polyclonal |
Sigma | A2052 | 1:1000 dilution |
| Antibody | Chicken anti-GFP polyclonal | Life Tech. | A10262 | 1:1000 dilution |
| Antibody | Rabbit anti-HA polyclonal | Cell sig. | 3724S | 1:500 dilution |
| Antibody | Rabbit anti-GFP polyclonal | Thermo Fisher S. | A11122 | 1:1000 dilution |
| Antibody | Rabbit anti-Unc-4 polyclonal | PMID:24550109 | 1:1000 dilution | |
| Antibody | Rabbit Eve | PMID:2884106 | 1:2000 dilution | |
| Antibody | Mouse anti-Acj6 monoclonal | DSHB | Acj6 | 1:100 dilution |
| Antibody | Rat anti-Cadherin monoclonal | DSHB | DN-Ex#8 | 1:25 dilution |
| Antibody | goat anti-rabbit Alexa Fluor 488 | Life Technologies | A-11034 | 1:500 dilution |
| Antibody | goat anti-rabbit Alexa Fluor 568 | Life Technologies | A-11011 | 1:500 dilution |
| Antibody | goat anti- rabbit Alexa Fluor 633 |
Life Technologies | A-21070 | 1:500 dilution |
| Antibody | goat anti-chicken Alexa Fluor 488 | Life Technologies | A-11039 | 1:500 dilution |
| Antibody | goat anti-mouse Alexa Fluor 568 | Life Technologies | A-11004 | 1:500 dilution |
| Antibody | goat anti-mouse Alexa Fluor 633 | Life Technologies | A-21050 | 1:500 dilution |
| Antibody | goat anti-rat Alexa Fluor568 | Life Technologies | A-11077 | 1:500 dilution |
| Antibody | goat anti-rat Alexa Fluor633 | Life Technologies | A-21094 | 1:500 dilution |
| Genetic reagent (D. melanogaster) | MKRS, P{ry[+t7.2]=hsFLP}86E/TM6B, P{w[+mC]=Crew}DH2, Tb[1] | BloomingtonDrosophila Stock Center | RRID:BDSC_1501 | |
| Genetic reagent (D. melanogaster) | P{ry[+t7.2]=hsFLP}1, w[1118]; Adv[1]/CyO | BloomingtonDrosophila Stock Center | RRID:BDSC_6 | |
| Genetic reagent (D. melanogaster) | y1 w*; P{UAS-FLP.D}JD1 | BloomingtonDrosophila Stock Center | RRID:BDSC_4539 | |
| Genetic reagent (D. melanogaster) | w*; P{UAS-p35.H}BH1 | BloomingtonDrosophila Stock Center | RRID:BDSC_5072 | |
| Genetic reagent (D. melanogaster) | P{Tub-dVP16AD.D} | BloomingtonDrosophila Stock Center | RRID:BDSC_60295 | |
| Genetic reagent (D. melanogaster) | P{Tub-GAL4DBD.D} | Bloomington Drosophila Stock Center | RRID:BDSC_60298 | |
| Genetic reagent (D. melanogaster) | w[*]; P{w[+mC]=iav-GAL4.K}3 | BloomingtonDrosophila Stock Center | RRID:BDSC_52273 | |
| Genetic reagent (D. melanogaster) | y[1] w[*]; Mi{y[+mDint2]=MIC}TfAP-2[MI04611]/TM3, Sb[1] Ser[1] | BloomingtonDrosophila Stock Center | RRID:BDSC_37965 | |
| Genetic reagent (D. melanogaster) | 13XLexAop2-IVS-myr::GFP in attP40 | BloomingtonDrosophila Stock Center | RRID:BDSC_32210 | |
| Genetic reagent (D. melanogaster) | w[*]; P{w[+mC]=tubP-GAL80[ts]}2/TM2 | BloomingtonDrosophilaStock Center | RRID:BDSC_7017 | |
| Genetic reagent (D. melanogaster) | hsFlp2::PEST;; HA_V5_FLAG-MCFO | PMID:25964354 | RRID:BDSC_9494 | |
| Genetic reagent (D. melanogaster) | y1 w*; P{ato-GAL4.3.6}10 | PMID:10774724 | ||
| Genetic reagent (D. melanogaster) | Dpn-EE-GAL4-attp16;T3/T6b | PMID:26700685 | ||
| Genetic reagent (D. melanogaster) | pJFRC29-10XUAS-IVS-myr::GFP-p10 in attP40 | PMID:22493255 | ||
| Genetic reagent (D. melanogaster) | pJFRC105-10XUAS-IVS-nlstdTomatoi n VK00040 | PMID:22493255 | ||
| Genetic reagent (D. melanogaster) | Poxn-GAL4.13 | PMID:12421707 | ||
| Genetic reagent (D. melanogaster) | y[1] w[*]; Mi{y[+mDint2]=MIC}TfAP-2[MI04611]/TM3, Sb[1] Ser[1] | BloomingtonDrosophilaStock Center | RRID:BDSC_37965 | |
| Genetic reagent (D. melanogaster) | UAS-hidAla5 | PMID:9814704 | ||
| Genetic reagent (D. melanogaster) | Actin5Cp4.6>dsFRT > nlsLexAp65 | G Rubin | ||
| Genetic reagent (D. melanogaster) | pJFRC51-3XUAS-IVS-Syt::smGFP-HA in attP40 | G Rubin | ||
| Genetic reagent (D. melanogaster) | pJFRC12-10XUAS-IVS-myr::GFP in attP18 | G Rubin | ||
| Genetic reagent (D. melanogaster) | unc-4-GAL4 | this study | FBgn0024184 | built by H. Lacin |
| Genetic reagent (D. melanogaster) | unc-4-AD | this study | FBgn0024184 | built by H. Lacin |
| Genetic reagent (D. melanogaster) | unc-4-DBD | this study | FBgn0024184 | built by H. Lacin |
| Genetic reagent (D. melanogaster) | unc-4-FRT | this study | FBgn0024184 | built by H. Lacin |
| Genetic reagent (D. melanogaster) | unc-4-null | this study | FBgn0024184 | built by H. Lacin |
| Genetic reagent (D. melanogaster) | TfAP-2-AD | this study | FBgn0261953 | built by H. Lacin |
| Genetic reagent (D. melanogaster) | TfAP-2-GAL4 | this study | FBgn0261953 | built by H. Lacin |
| Genetic reagent (D. melanogaster) | w*;sca-GAL4 | PMID:2125959 | ||
| Chemical compound, drug | Paraformaldehyde | EMS | 15713 | |
| Chemical compound, drug | Vectashield | Vector | H-1000 | |
| Chemical compound, drug | Prolong Diamond | Molecular Probes | P36961 | |
| Recombinant DNA reagent | pHD-DsRed-attP | Addgene | 51019 | |
| Recombinant DNA reagent | pCFD4-U6:1_U6:3tandemgRNAs | Addgene | 49411 | |
| Recombinant DNA reagent | pBS-KS-attB2-SA(1)-T2A-Gal4-Hsp70 | Addgene | 62897 | |
| Recombinant DNA reagent | pBS-KS-attB2-SA(1)-T2A-Gal4DBD-Hsp70 | Addgene | 62903 | |
| Recombinant DNA reagent | pBS-KS-attB2-SA(1)-T2A-p65AD-Hsp70 | Addgene | 62914 | |
| Recombinant DNA reagent | pBS-KS-attB2-SA(0)-T2A-Gal4-Hsp70 | Addgene | 62896 | |
| Recombinant DNA reagent | pBS-KS-attB2-SA(0)- T2A-Gal4DBD-Hsp70 |
Addgene | 62902 | |
| Recombinant DNA reagent | pBS-KS-attB2-SA(0)-T2A-p65AD-Hsp70 | Addgene | 62912 |