Table 1. Three-leg rubbing and climbing behavior in response to tissue specific removal of Unc-4.
Success in three-leg rubbing | Success in climbing, time to climb§ (in seconds) | ||||
---|---|---|---|---|---|
Driver | Target cell types | Control | unc-4 removal | Control | unc-4 removal |
sca-GAL4 | All CNS and PNS neurons | 88.9%, N = 18 | 0%*, N = 20 | 100%, 17.7 s. +/- 8, N = 22 | 9.1%*, N/A¶, N = 22 |
dpn-GAL4 | All CNS neurons | 81.8%, N = 22 | 28.5%*, N = 21 | 90%, 20.1 s. +/- 15, N = 30 | 80%†, 27.6 s.‡+/- 20.5, N = 28 |
ato-Gal4 | Chordotonal neurons | 90%, N = 33 | 14.8%*, N = 27 | N/A** | N/A** |
*Significant change, Fisher exact test P value < 0.001.
†Non-significant change, Fisher exact test p value>0.1.
‡Non-significant change, Mann-Whitney U Test p value>0.1.
§failed climbing attempts not included for quantification.
¶ not assessed since most animals failed to climb.
**ato-GAL4 >FLP expression impaired the negative geotaxis in both control and experimental animals, thus climbing assay was not performed.