Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Saccharomycetales) | Flo11-type A domain sequences | various | See Supplementary file 1 | |
| Genetic reagent Saccharomyces cerevisiae | Flo11-type A domain variants carrying yeast strains | See Supplementary file 2 | ||
| Antibody | Rabbit polyclonal anti-ScFlo11A | (Kraushaar et al., 2015) | Previously produced in our lab (1:10.000) |
|
| Antibody | Mouse monoclonal anti-STREP II Chromeo 488 | IBA Lifesciences, Göttingen, Germany | Cat# 2-1544-050 | (1:1.000) |
| Recombinant DNA reagent | Flo11-type A domain variants carrying plasmids | various | See Supplementary file 3 | |
| Sequence-based reagent | Primer Ampl_FLO11_wt_fw | This study | PCR primers | CTC AAA AAT CCA TAT ACG CAC ACT |
| Sequence-based reagent | Primer Ampl_FLO11_wt_BC_rev |
This study | PCR primers | GTA GAG CTG GAT GGA GTT GG |
| Peptide, recombinant protein | ScFlo11A | (Kraushaar et al., 2015) | Previously produced in our lab | |
| Peptide, recombinant protein | KpFlo11A | This study | ||
| Commercial assay or kit | Amine coupling kit | GE Healthcare, Solingen, Germany |
||
| Software, algorithm | Matlab | MathWorks | RRID:SCR_001622 | See section ‘QCAM’ and ‘Competitive biofilm assay’ |
| Software, algorithm | GraphPad Prism, v7.0 | GraphPad Software, La Jolla, CA, USA |
RRID:SCR_002798 | |
| Software, algorithm | XDS | (Kabsch, 2010) | RRID:SCR_015652 | |
| Software, algorithm | CCP4 | Collaborative Computational Project, Number 4, 1994 | RRID:SCR_007255 | |
| Software, algorithm | Phenix suite | (Adams et al., 2002) | RRID:SCR_014224 | |
| Software, algorithm | Coot | (Emsley et al., 2010) | RRID:SCR_014222 | |
| Software, algorithm | Refmac5 | (Murshudov et al., 2011) | RRID:SCR_014225 | |
| Software, algorithm | PyMOL v1.8.0.1 | (Delano, 2002) | RRID:SCR_000305 | |
| Software, algorithm | Biacore T100 evaluation software, v1.1 | GE Healthcare, Solingen, Germany | ||
| Software, algorithm | Clustal Omega web tool | (McWilliam et al., 2013) | RRID:SCR_001591 | |
| Software, algorithm | PRANK web tool | (Löytynoja and Goldman, 2010) | RRID:SCR_017228 | |
| Software, algorithm | FigTree v1.4.2 | http://tree.bio.ed.ac.uk | RRID:SCR_008515 | |
| Software, algorithm | STRIDE web server | (Heinig and Frishman, 2004) | ||
| Software, algorithm | Modeller 9v7 | (Sali and Blundell, 1993) | RRID:SCR_008395 |