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. 2020 Apr 10;3(5):e202000661. doi: 10.26508/lsa.202000661

Figure S1. Plasmid construction and validation.

Figure S1.

(A) Wild-type Vav1 was subcloned into Teto7minCMV plasmid transgene plasmid fused to GFP (tetO–Vav1). (B) HEK293 cells were transfected with the tetO–Vav1 plasmid and a vector that encodes the reverse tetracycline responsive transactivator (rtTA). After treatment with Dox, the cells were lysed and analyzed by Western blotting for the presence of Vav1 and its phosphotyrosine status. The empty plasmid served as control vector. (C) HEK293 cells were transfected with the tetO–Vav1 plasmid either treated (+) or nontreated (−) with Dox were subjected to immunofluorescence. Green = GFP, which refers to Vav1 expression. Scale bar represents 50 μm.