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. 2020 Apr 13;3(5):e201900535. doi: 10.26508/lsa.201900535

Figure 4. Bmal1−/− mouse embryonic stem cells (mESCs) display reduced induction of endoderm and mesoderm genes during monolayer cell differentiation.

Figure 4.

(A, B, C) Scheme of the protocol used to analyse differentiation of naïve pluripotent cells in (B, C). (B, C) mRNA expression of pluripotency (black), ectoderm (blue), endoderm (green), and mesoderm (red) specific genes, at indicated days during differentiation as measure by RT-qPCR in JM8 wild-type and Bmal1−/− (grey) mESCs. Expression was normalized to housekeeping genes Hmbs and Ywhaz. Fold change relative to time 0 is represented. (D, E, F) Scheme of the protocol used to analyse differentiation of primed serum pluripotent cells in (E, F). (E, F) mRNA expression of pluripotency (black)-, ectoderm (blue)-, endoderm (green)-, and mesoderm (red)-specific genes at indicated days during differentiation as a measure by RT-qPCR in JM8 wild-type and Bmal1−/− (grey) mESCs. Expression was normalized to housekeeping genes Hmbs and Ywhaz. Fold change relative to time 0 is represented. (B, C, E, F) Mean and SEM of three independent experiments are shown in (B, C, E, F). (B, C, E, F) Asterisks (*) in (B, C, E, F) indicate significant difference (T-student P < 0.05) between Bmal1−/− and JM8 parental cells at the same time point.