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. Author manuscript; available in PMC: 2021 Mar 9.
Published in final edited form as: Curr Biol. 2020 Feb 20;30(5):802–814.e8. doi: 10.1016/j.cub.2019.12.067

Figure 2. Identification of the RTO gene in Mimulus lewisii and M. guttatus and its relative expression in various mutant and transgenic lines.

Figure 2.

(A–C) Bulked segregant analyses of rto in M. lewisii (A) and rtoSWC (B) and rtoLRD (C) in M. guttatus narrowed RTO down to the same genomic interval. (D) Schematic of the RTO (R3-MYB) gene, showing the molecular lesions of the five mutant alleles. (E and F) Relative transcript level of RTO (upper) and NEGAN (lower) in M. lewisii (E) and M. guttatus (F) as measured by qRT-PCR, standardized to the corresponding wild-type (LF10 for M. lewisii, SWC for M. guttatus). Error bars represent 1 SD from three biological replicates. See also Figures S3 and S4 and Tables S4 and S6.