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. Author manuscript; available in PMC: 2021 Mar 9.
Published in final edited form as: Curr Biol. 2020 Feb 20;30(5):802–814.e8. doi: 10.1016/j.cub.2019.12.067

Figure 4. CRISPR/Cas9 mediated knockout of MgRTO in Mimulus guttatus.

Figure 4.

(A) Flower phenotypes of three independent CRISPR knockout lines in their heterozygous (+/−) and homozygous (−/−) conditions, compared to the wild-type (MAC; +/+). (B) Complementation crosses between a CRISPR allele (rtoCRISPR−36) and two natural mutant alleles (rtoLRD and rtoQVR). (C) Examples of six independent CRISPR alleles relative to gRNA position in MgRTO. The primer pair 5’-GTCTTCATATATTCCCATCTCTT-3’ and 5’-CGTGCTCGGTGTAAGTAACG-3’ was used for amplifying and sequencing the target region. See also Figure S2 and Table S5.