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. 2020 Apr 14;10:5646. doi: 10.1038/s41598-020-62286-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Histological analysis of perfused liver tissues. (a–c) Appearances of the liver tissues constructed under different conditions as follows: non-perfused condition with a normal density of mesenchymal stem cells (MSCs) (a), perfused condition with a low density of MSCs (b), and perfused condition with the normal density of MSCs (c). HUVECs, human umbilical vein endothelial cells. Asterisks indicate the main channels. (d–f) HE-stained transverse sections of the liver tissues. (g–i) Immunofluorescent images in which cells were stained for nuclei (cyan), CD31 (green), and EpCAM (magenta).