Figure 3.

Western blot analysis of EGFR-dependent signalling pathways in TNBC cell lines treated with gefitinib and everolimus. Western blot analysis with the indicated antibodies was performed as described in the materials and methods section using 20 µg of whole cell protein extract. Cell lines were exposed for 24 h to 5 µM gefitinib (GEF) and/or 100 nM everolimus (EVERO). Beta-actin antibody was used as a loading control. Cropped blots from different gels are grouped together for clear illustration. The full-length gels are shown in the Supplementary Information. Data shown are representative of two independent experiments.