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. 2020 Apr 14;10:6361. doi: 10.1038/s41598-020-63417-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HIF-1α silencing abolishes HT-dependent GLUT-1 upregulation but not AM and VEGF induction. (a) Representative immunoblot of HIF-1α knockdown with different concentrations of siHIF-1α. Effect of HIF-1α –silencing (siHIF-1α 40 nM) on the transcription level of (b) GLUT-1, (c) AM and (d) VEGF in hypoxic cells after treatment with HT 100 and 200 μM. Results are expressed as mRNA levels relative to Scr hypoxic cells after normalization against PPIA. Values represent the mean ± SD from three independent experiments. Statistically significant differences with the corresponding Scr-siRNA transfected hypoxic cells: ^ap < 0.05, ^aap < 0.01. Statistically significant differences with the corresponding HIF-1α-silenced cells: ^bp < 0.05, ^bbp < 0.01. Statistically significant differences with the corresponding HT treated-non-silenced cells: ^cp < 0.05, ^ccp < 0.01, ^cccp < 0.001.