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. 2020 Apr 14;11:1827. doi: 10.1038/s41467-020-15552-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Immunoblot analysis of the C-terminal domain of RNA polymerase II, total (Rpb1) and phosphorylated (p-Rpb1(S2)), Cyclin T1 and CDK9 protein expression in wild-type heart and liver isolated from 15-day-old (15d) and 60-day-old (60d) mice. Replicate samples are derived from independent mice. b Quantitative RT-PCR analysis of Cad, Bzw2, Pinx1, Polr3d, St6 and Cdc25a expression in wild-type (R26^+/+) and R26^CMER/+ heart isolated from 15-day-old (15d, Bzw2 and Cad n = 6, Cdc25a, Pinx1, Polr3d and St6 n = 7) mice vs heart (Bzw2, R26^+/+ Pinx1 and R26^CMER/+ Cad n = 9, Cdc25a, Polr2d, St6, R26^+/+ Cad and R26^CMER/+Pinx1 n = 8, R26^CMER/+ St6 n = 7, R26^CMER/+ Cdc25a, Polr3d n = 6) and liver (R26^+/+ Bzw2, Pinx1, Cad, Cdc25a and St6 n = 9, R26^+/+ Polr2d n = 8, R26^CMER/+ Cad n = 10, R26^CMER/+ Bzw2 n = 8, R26^CMER/+ St6 n = 7, R26^CMER/+ Cdc25a, Pinx1, Polr3d n = 6) isolated from 60-day-old (60d) mice 4 h post administration of 4-OHT. Expression is relative to the respective wild type (R26^+/+). Mean and s.d shown. One-way ANOVA with Tukey’s multiple comparisons test; 15 day R26^+/+ 4-OHT vs R26^CMER/+ 4-OHT: ***P = 0.001 (Cad, Bzw2, Pinx1, St6 and Cdc25a), 15 day R26^+/+ 4-OHT vs R26^CMER/+ 4-OHT: **P = 0.001 (Polr3d), adult heart R26^+/+ 4-OHT vs R26^CMER/+ 4-OHT: ***P = 0.001 (Cad), adult liver R26^+/+ 4-OHT vs R26^CMER/+ 4-OHT: ***P = 0.001 (Cad, Bzw2, Pinx1, St6 and Cdc25a). Replicate samples are derived from independent mice. c Heatmap showing the union of DEGs called in each tissue; adult liver (n = 3), adult heart (n = 5) and 15-day-old (15d) heart. mRNA expression fold changes (Log2) upon MycER activation are shown relative to wild type, as determined by RNA sequencing of R26^CMER/+ (n = 3) tissues relative to wild type (R26^+/+, n = 3) at 4 h post administration of 4-OHT. d Quantification of p-H3-positive nuclei percentage in heart (positive cardiomyocyte nuclei only) and liver (positive hepatocyte nuclei only) isolated from 15-day-old (15d) and 60-day-old (60d) wild-type (R26^+/+, n = 10 15d, n = 8 60d) and R26^CMER/+ (n = 4) mice 24 h post administration of tamoxifen. Means are taken from five images per mouse; mean and s.d. shown. One-way ANOVA with Tukey’s multiple comparisons test; R26^+/+ 4-OHT vs R26^CMER/+ 4-OHT: ***P = 0.001 (15-day heart and adult liver). Source data are provided as a Source Data file.