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. 2020 Apr 14;11:1827. doi: 10.1038/s41467-020-15552-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a Immunohistochemistry (left) and quantification (right) of Cyclin T1 in the liver isolated from adult R26^CMER/+ or heart isolated from Myh6-Cre; R26^LSL-CMER/+ mice systemically infected with adeno-associated virus (serotype 9, 1 × 10¹¹ vg/mouse) encoding either RFP (AAV9-RFP, n = 5) or Ccnt1 (AAV9-Ccnt1, n = 4) 4 weeks post infection. Mean and s.e.m shown. Unpaired t-test; AAV9-RFP vs AAV9-Ccnt1: ***P < 0.0001. Representative images based on analysis of five independent mice. b Immunoblot analysis of the C-terminal domain of phosphorylated RNA polymerase II (p-Rpb1(S2)), CDK9 and Cyclin T1 expression in the heart isolated from adult Myh6-Cre; R26^LSL-CMER/+ mice systemically infected with adeno-associated virus encoding either RFP (AAV9-RFP) or Ccnt1 (AAV9-Ccnt1) 4 weeks post infection. Replicate samples are derived from independent mice. c Quantitative RT-PCR analysis of Cad, Bzw2, Pinx1, Polr3d, St6 and Cdc25a expression in control (R26^LSL-CMER/+; no cre, n = 5) and Myh6-Cre; R26^LSL-CMER/+ (R26^LSL-CMER/+; cre+, n = 7) adult mouse heart isolated 4 weeks post systemic infection with an adeno-associated virus encoding Ccnt1 (AAV9-Ccnt1) and 4 h post administration of 4-OHT. Expression is relative to the respective wild type (R26^LSL-CMER/+). Mean and s.e.m shown. Unpaired t-test; no cre vs cre+: ***P < 0.0005 (Cad, Bzw2, Pinx1 and St6), **P = 0.0013 (Cdc25a), **P = 0.0035 (Polr3d). Replicate samples are derived from independent mice. d Enrichment of common Myc targets (liver, lung, kidney and heart) in cardiomyocytes isolated from the heart of AAV-CCNT1 infected Myh6-cre; R26^LSL-CMER/+ (Myc+Cyclin T1) mice in comparison to uninfected Myh6-cre; R26^LSL-CMER/+ mice (Myc) at 4 h post administration of 4-OHT, as determined by RNA sequencing. Including normalised enrichment score (NES) and FDR q-value (FDR).