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. 2020 Apr 14;11:1827. doi: 10.1038/s41467-020-15552-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 9 — a Immunofluorescent staining of cardiac troponin, p-H3,Ki67, PCM1, wheat germ agglutinin (WGA) and aurora B in control (R26^LSL-CMER/+; no cre) and Myh6-Cre; R26^LSL-CMER/+ (R26^LSL-CMER/+; cre+) adult mouse heart isolated 4 weeks post systemic infection with an adeno-associated virus encoding Ccnt1 (AAV9-Ccnt1) and 48 h post administration of tamoxifen. Representative images based on analysis of five independent mice. Scale bars represents 10 μm (aurora B) and 50 μm (all others). b Quantification of p-H3-positive nuclei percentage in control (R26^LSL-CMER/+; no cre, n = 9) and Myh6-Cre; R26^LSL-CMER/+ (R26^LSL-CMER/+; cre+, n = 7) adult mouse heart isolated 4 weeks post systemic infection with an adeno-associated virus encoding Ccnt1 (AAV9-Ccnt1) and 48 h post administration of tamoxifen. Means are taken from five images per mouse; Mean and s.e.m shown. Unpaired t-test; no cre vs cre+ P < 0.0001. c The weight (mg) of hearts isolated from control (R26^LSL-CMER/+; no cre, n = 9 48 h, n = 5 72 h) and Myh6-Cre; R26^LSL-CMER/+ (R26^LSL-CMER/+; cre+, n = 7 48 h, n = 6 72 h) adult mouse heart 4 weeks post systemic infection with an adeno-associated virus encoding Ccnt1 (AAV9-Ccnt1) and 48 and 72 h post administration of tamoxifen, expressed as fold change over the length (mm) of a tibia isolated from the same mouse. Mean and s.e.m shown. One-way ANOVA with Tukey’s multiple comparisons test; no cre vs cre+: *P = 0.05 (48 h), ***P = 0.001 (72 h). Replicate samples are derived from independent mice. d Image of the whole heart and a tibia from control (R26^LSL-CMER/+; no cre) and Myh6-Cre; R26^LSL-CMER/+ (R26^LSL-CMER/+; cre+) adult mouse heart isolated 4 weeks post systemic infection with an adeno-associated virus encoding Ccnt1 (AAV9-Ccnt1) and 72 h post administration of tamoxifen. Scale bar represents 10 mm. Representative images based on analysis of 11 independent mice. e Quantification of the number and size of cardiomyocytes from control (R26^LSL-CMER/+; no cre, n = 5) and Myh6-Cre; R26^LSL-CMER/+ (R26^LSL-CMER/+; cre+, n = 6) adult mouse heart isolated 4 weeks post systemic infection with an adeno-associated virus encoding Ccnt1 (AAV9-Ccnt1) and 72 h post administration of tamoxifen. Mean and s.d shown. One-way Mann–Whitney test; no cre vs cre+ **P = 0.0043. Replicate samples are derived from independent mice. Source data are provided as a Source Data file.