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. 2020 Mar 24;52(3):514–528. doi: 10.1038/s12276-020-0406-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a BMMs transfected with control or DCTN1 siRNA were cultured in ODM for 4 days and stained for TRAP. TRAP-positive multinucleated cells were counted. b BMMs transfected with control or DCTN1 siRNA were cultured in ODM for the indicated number of days. NFATc1, c-Fos, and DCTN1 protein levels were determined by western blotting. c BMMs transfected with control or DCTN1 siRNA were cultured with ODM for 48 h. DCTN1, NFATc1, c-Fos, and Ctsk mRNA levels were analyzed by real-time PCR. d BMMs transfected with control or DCTN1 siRNA were cultured on dentin slices in ODM for 9 days. The black areas indicate the resorbed surfaces on dentin slices. The resorption area and pit depth were analyzed by confocal microscopy. e BMMs transfected with control or DCTN1 siRNA were cultured in ODM for 4 days. The cells were stained with Alexa Fluor 633-conjugated phalloidin. *p < 0.05, **p < 0.005 compared with controls.