Fig. 1. GNF179-resistant yeast strains harbor mutations in endoplasmic reticulum(ER)-based lipid homeostasis and autophagy genes.

a Protein maps showing relevant mutations and PROSITE predicted protein domains, if applicable. Maps were generated using Illustrator of Biological Sequences (IBS) software package⁸⁶. Missense mutations are shown in yellow ovals, nonsense mutations in red pentagons, and frameshift mutations as purple arrows. b Protein–protein interaction (PPI) network was generated using the STRING database⁸⁷. Each node represents a S. cerevisiae protein and connecting lines delineate interactions. The PPI enrichment p-value (p = 1.38  × 10⁻¹⁴) indicates that the proteins show significantly more interactions among themselves than would be expected from a random subset of genes from the yeast genome.