Table 2.
IC50s (mean and S.E.M) for GNF179 in the indicated yeast CRISPR-Cas9 edited strains.
| Strain name | Systematic gene ID | Standard gene name | Mutation | Gene description | GNF179 IC50 (µM) | GNF179-fold resistance (FR) |
|---|---|---|---|---|---|---|
| ABC16-Monster | N/A | N/A | N/A | N/A | 47.30 ± 3.8 | N/A |
| EAW361 | YBR171W | SEC66 | M1I | Non-essential subunit of Sec63 complex, involved in protein targeting and import into the ER | 70.10 ± 11.7 | 1.5 |
| EAW277 | YBR171W | SEC66 | S107* | Non-essential subunit of Sec63 complex, involved in protein targeting and import into the ER | 74.83 ± 9.4 | 1.6 |
| EAW289 | YCR034W | ELO2 | G183C | Fatty acid elongase, involved in sphingolipid biosynthesis; | 88.15 ± 8.7 | 1.9 |
| EAW327 | YLR372W | ELO3 | Y307* | Fatty acid elongase, involved in sphingolipid biosynthesis; | 76.57 ± 10.0 | 1.6 |
Strain name, systematic name, 18 h IC50s against GNF179 (mean ± standard error with n ≥ 3) and relative change compared to unedited ABC16-Monster strain are indicated.