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. 2020 Mar 9;52(3):438–449. doi: 10.1038/s12276-020-0393-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Comparison of gross cell morphology of ASCs with/without miR-150 transfection. There was no difference in gross cell morphology between the two groups. b Flow cytometric analysis of ASCs transfected with miR-150. The miR-150-transfected ASCs were negative for CD31 and CD45 (hematopoietic stem cell markers) and positive for CD73 and CD105 (mesenchymal stem cell markers), and the expression pattern in ASCs without miR-150 transfection was identical to that of transfected ASCs. c Successful adipogenic [Top] and osteogenic [Bottom] differentiation of miR-150-transfected ASCs. Adipogenic and osteogenic differentiation were identified using Oil Red O and Alizarin red stains, respectively. d Successful chondrogenic differentiation of miR-150-transfected ASCs. Chondrogenic differentiation was identified using collagen types 1 and 2 and proteoglycan stains. Scale bars = 100 µm. The values are presented as the mean ± standard deviation of three independent experiments. *P < 0.05. ASC adipose-derived stem cell, t-ASC miR-150-transfected adipose tissue-derived stem cell.