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. 2020 Apr 14;10:6264. doi: 10.1038/s41598-020-63226-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Vitellogenin mRNA expression pattern in the ZFL cells post-PNX-20 treatment (A-L). The left panel represents 1 and 2 hr and the right panel represents 6 and 24 hr treatment groups. No significant changes in any of the vitellogenin mRNA expression was observed in 1 hr treatment groups (1hr- A,C,E,G,I,K). Among 2 hr treatment groups, vtg 1, vtg 3, vtg 4, vtg 5 and vtg 7 mRNA expression was upregulated (2 hr- A,E,G,I,K). However, significant upregulation of all vtg’s was observed in the 6 and 24 hr treatment groups (B,D,F,H,J,L). Graphs represent pooled data from 2 independent study (‘n’ = 6 wells/treatment). One-way ANOVA followed by Tukey’s multiple comparison test or Student–Newman–Keuls (SNK) test was used for statistical analysis. Asterisks denote significant differences between control and treated groups. p < 0.05 was considered statistical significance.