Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 14;11(4):231. doi: 10.1038/s41419-020-2427-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a B16F10 cells were transiently transfected with pCMV-Myc-N (empty vector, EV) or Myc-HopQ for 12 h, then treated with 40 μg/ml mitomycin C for 2 h and scratch wound-healing assays were performed. Bar graphs show the percentage of cell-covered area. (***P ≤ 0.001). Scale bar: 250 μm. b, c EV- or Myc-HopQ-expressing B16F10 were seeded in transwell chambers and incubated for 24 h. The lower chamber containing 10% FBS was used as a chemoattractant. For the cell invasion assay, the membrane was coated with 10 mg/ml of Matrigel. Bar graphs show the percentage of migrating or invading cells (***P ≤ 0.001). Scale bar: 200 μm. d Live-cell tracking of the movement of ten single EV and Myc-HopQ B16F10 cells. Live-cell motility of B16F10 was monitored every 20 min over a 11-h period. e B16F10 cells were transfected with EV or Myc-HopQ for 12 h, and cell viability was measured via the CCK8 assay at the indicated time. NS: no significant difference. f B16F10 cells were transfected with EV or Myc-HopQ for 12 h, re-plated on 6-well plates, and cultured for 7 d. The cells were fixed and stained with 0.1% Crystal Violet. NS no significant difference.