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. 2020 Apr 14;11(4):231. doi: 10.1038/s41419-020-2427-y

Fig. 3. HopQ binds to vimentin and inhibits its stability.

Fig. 3

a B16F10 cells were transfected with increasing doses of Myc-HopQ for 24 h, and cell lysates were collected for immunoblot. b EV- or Myc-HopQ-transfected B16F10 cells were analyzed by RT-PCR with vimentin-specific primers. NS no significant difference. c B16F10 cells were transfected with Myc-HopQ for 16 h and treated with cycloheximide (CHX) 10 μM at the indicated time points. Cell lysates were then collected for immunoblot. d Quantification of the relative levels of vimentin after the treatments described in c. (**P ≤ 0.01). The amount of vimentin was normalized to the β-actin level. e B16F10 cells were transfected with Myc-HopQ, and whole-cell lysates were used for IP with anti-Vimentin antibody and analyzed by immunoblot with anti-Myc antibody. Arrowheads indicate vimentin bands. f B16F10 cells were transfected with Myc-HopQ or its mutants for 24 h. Whole-cell lysates were then harvested for IP with anti-Vimentin antibody and immunoblot analysis with anti-Myc antibody.