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. 2020 Apr 14;11(4):231. doi: 10.1038/s41419-020-2427-y

Fig. 6. HopQ induces the interaction between vimentin and p62 for selective autophagic degradation and inhibits melanoma metastasis.

Fig. 6

a B16F10 cells were transfected with EV or Myc-HopQ and treated with 10 nM bafilomycin A1 (BafA1) for 12 h, then cell lysates were used for IP with anti-Myc antibody and analyzed by immunoblot with anti-Ubiquitin antibody. b, c Total lysates of B16F10 cells were transfected with EV or Myc-HopQ, IP with anti-Myc (b) or anti-Vimentin (c) antibody and analyzed by immunoblot with anti-p62 antibody. d EV or Myc-HopQ-expressing B16F10 cells were injected into 8-week-old male C57BL/6 mice (n = 5) through the tail vein. The lungs with metastasis were visually counted within 2 weeks after tail vein injection of cells. Representative images of lung metastasis. e Quantification of metastatic lung nodules. (*P ≤ 0.05). f Representative images of hematoxylin and eosin (H&E)-stained lung tissue sections. Scale bar: 200 μm. g Number of lung nodules in H&E-stained lung sections in EV (N = 5) and Myc-HopQ (N = 5) groups. (*P ≤ 0.05). h Proposed model for HopQ-mediated selective autophagic degradation of vimentin in melanoma cells.