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. 2020 Apr 14;10:6420. doi: 10.1038/s41598-020-62934-8

Figure 2.

Figure 2

QAC18 induced a cyst-like morphology in A. castellanii. Acanthamoeba trophozoites (105 cells/ml) incubated without (A) with QAC18 at 37.5 µg/ml for 1 h at 25 °C (B) and 0.5% SDS for 30 min (C). QAC18 reduced trophozoites sizes from 29.02 ± 0.23 (A) to 7.87 ± 0.93 (B) and disintegrated after 30 min treatment with SDS (C). Distribution of the area (µm2) (D), optical volume (µm3) (E) and perimeter length (µm) (F) of trophozoites treated for 24 h with (grey bar) and without (black bar) QAC18 assessed using the Holomonitor M4.