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. 2020 Apr 14;10:6420. doi: 10.1038/s41598-020-62934-8

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© The Author(s) 2020, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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QAC-DNA interaction. The absorbance of genomic DNA (0.6 µg/ml) extracted from A. castellanii trophozoites measured at 260 nm with the Nanodrop after incubating for 15 minutes with 0 μg/ml, 0.6 μg/ml, 6 μg/ml or 12 μg/ml of QAC12 (A) or QAC16 (B) to give ratios of 1:0, 1:1, 1:10 and 1:20. DNA absorbance increased and decreased with increased [QAC18] and [QAC12] respectively. Data is mean absorbance at 260 nm ± SD; n = 4 independent experiments performed in triplicates. Student’s t-test showed significant difference between mean absorbance at 1.1, 1:10 and 1:20 relative to 1:0 at p < 0.01 for QAC18 (A) and QAC12 (B) respectively.