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. 2020 Apr 14;10:6420. doi: 10.1038/s41598-020-62934-8

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© The Author(s) 2020, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extrusion of cytoplasmic constituents of A. castellanii cysts to the external milieu after QAC treatment. Protein (A) released after the incubation of 10⁵ A. castellanii cysts, with (QAC18) and without (control) QAC18 at 37.5 µg/ml for 24 h, measured in spent medium using the Bradford assay. Data is mean ± SD respectively (A); n = 4 independent experiments performed in triplicates. Student’s t-test showed significant difference between mean [proteins] in control and QAC18-treated experiments. **p < 0.01. (B) Extracellular proteins were visualised with SDS-PAGE. Key: Lane 1; Protein ladder, Lane 2; spent media form untreated cysts, Lane 3; spent medium from QAC18-treated cysts.