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. 2020 Mar 2;52(3):423–437. doi: 10.1038/s12276-019-0359-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a TEM images (×80,000) of EVs extracted from L. paracasei-grown media on day 5. The diameters (nm) of LpEVs measured by nanoparticle tracking analysis (NTA) on day 5 (b, c) in vivo fluorescence assay. LpEVs were labeled with Cy7-NHS, and 10 µg of Cy7-labeled LpEVs was administered by gavage to the mice. After administration, the fluorescent signal of the whole body or dissected organs was detected depending on the time. 1. brain, 2. blood, 3. heart, 4. lung, 5. liver, 6. stomach, 7. spleen, 8. small intestine, 9. kidney, 10. large intestine.